EUROPEAN CONGRESS AND EXHIBITION ON ADVANCED MATERIALS AND PROCESSES - EUROMAT 2021

The wetting behavior of surfaces strongly depends on their roughness. In this work we investigated the influence of surface roughness of Ti6Al4V alloy on the adhesion of HEK293 human cells. The array of linear scratches has been produced on the surface of cold-wrought Ti6Al4V alloy discs with the aid of various abrasive SiC papers at grades 220, 400, 600, 1000, 1200 and 2000 (number of grains per cm2) and different diamond pastes with grain sizes 6, 3 and 1 μm. These SiC papers grades correspond to the SiC grain size of 68-10 μm. The resulted surface topography of the samples was examined by the conventional and confocal light microscopy, atomic force microscopy and high-resolution scanning electron microscopy. Afterwards, the adhesion of the HEK293 human cells to the samples of different roughness has been measured. The portion of adhered cells behaves non-monotonously with grain size. It is about 0.35 for grain size of 1 μm, it increases with increasing grain size up to 0.95 for grain size of 7 μm and then slowly decreases down to 0.55 at abrasive grain size of 68 μm. Thus, the variation of surface roughness opens the way to control and tailor the potion of adhered cells, depending on demand of medical techniques.
This work was carried out with the financial assistance of the Russian Foundation for Basic Research (grant 19-58-06002) and Israel Ministry of Science and Technology (grant 3-16534).