EUROPEAN CONGRESS AND EXHIBITION ON ADVANCED MATERIALS AND PROCESSES - EUROMAT 2021

Mesoporous bioactive glass nanoparticles generated significant attention in recent years as suitable materials for biomedical application. In this study, cerium was incorporated into mesoporous bioactive glass nanoparticles (MBGNPs) using two different approaches. In the first approach, cerium was added to the glass system directly during the synthesis, while in the second approach cerium was added to the as-synthesized MBGNPs ex-post, via post-modification method. The influence of the method of synthesis method on physicochemical properties of nanoparticles was examined by SEM, TEM, XRD, FTIR, and N2 adsorption-desorption method. The MBGNPs have spheroidal and pineal shaped morphology and disordered mesoporous structure. XRD analysis confirmed the amorphous nature of the nanoparticles. The chemical composition was determined by the acid digestion method using ICP-OES. The influence of the synthesis method on the specific surface area, mesoporosity, and solubility of synthesized nanoparticles in Tris and Acetate buffer has been also studied. MC3T3-E1 cells viability after direct exposure to MBGNPs up to 500 ug/mL were evaluated over time using WST-8 assay. MBGNPs did not show any cytotoxicity towards the MC3T3-E1 cells up to 100 ug/mL. To investigate the cell viability further, the cells were stained with calcein-AM and propidium iodide which selectively stained live or dead cells. Additionally, Ce doped MBGNPs also showed antibacterial activity against S. aureus and E. coli. The mentioned features of the obtained cerium containing MBGNPs particles makes them useful for multifunctional applications as drug delivery carriers or bioactive fillers for bone tissue engineering applications with enhanced antibacterial effect.