Speaker
Description
Introduction:
Implant associated infections, commonly caused by biofilm-forming bacteria end in 70% implant rejections that are highly problematic for the patient, the healthcare system, and society. Poly(D,L-lactide-co-glycolide) (PLGA) and Poly(D,L-lactide) (PDLLA) are well studied and approved bioresorbable polymers (BRP) for biomedical applications [1]. Due to the antibiofouling properties of hydrophilic Polyethylene glycol (PEG) [2] and the broad-spectrum antibacterial applications of the Silver Sulfadiazine (AgSD) [3], we propose a novel AgSD-loaded co-polymer coating to prevent the biofilm related infections in dental and orthopaedic implants.
Methods:
Di-block co-polymers of PLGA-PEG and PDLLA-PEG were synthesized by ring opening polymerization of D,L Lactide, Glycolide and methyl-ether PEG 5000. The obtained co-polymers were characterized by H1-NMR and GPC. Methylene chloride was used to dissolve the co-polymers following the addition of AgSD, the dissolution was stirred for 1 hour and poured into glass covered 24 wells plate for 48 hours solvent evaporation, creating a film cast. With our AgSD-loaded co-polymers attached to the well plate, we proceed with the preliminary Violet crystal assay as per manufacturer specifications using Pseudomonas, S. Aureus and S. Epidermis, with the quantification of cell viability in microplate reader.
Results:
Confirmation of the polymerization and mPEG presence was made by H1-NMR and GPC analysis found a suitable molecular weight and unimodal peak confirmation for di-block structure. The addition of PEG plus AgSD showed a significant decrease in proliferation and adherence of the bacterial strains to the polymer coating in comparison to the control group of PLGA and PDLLA without PEG and AgSD.
| Speaker Country | Ireland |
|---|