EUROPEAN CONGRESS AND EXHIBITION ON ADVANCED MATERIALS AND PROCESSES - EUROMAT 2021

In recent years, antimicrobial resistance has become one of the main threats to global health. This drawback lies mainly in the formation of biofilms, which are a defense mechanism of microorganisms against external aggressions, including antibiotics [1]. In addition, it has been shown in previous studies that severe infection leads to changes in factors such as vascular endothelial growth factor (VEGF) [2] which plays a key role in initiating the process of angiogenesis [3].
Herein we have developed a multicomponent nanocarrier based on mesoporous silica nanoparticles (MSN) to combat bacterial biofilm and also help angiogenesis during bone tissue regeneration process. The nanosystem has been designed bearing polycationic dendrimers in the external surface (MSN-G3) to favor internalization in bacteria [1]. Cu2+ cations with both bactericidal and angiogenic effect have been anchored to the dendritic branches by complexation with the amino groups of the dendrimer (MSN-G3-Cu2+), and a broad-spectrum antibiotic such as levofloxacin has been incorporated into the mesopores.
In vitro antimicrobial assays performed with E. coli and S. aureus bacteria in planktonic and biofilm state have shown that MSN-G3-Cu2+ exhibit antibacterial behavior in Gram negative and positive bacterial models. In addition, a combined effect was found when the nanosystem incorporates the antibiotic. Biocompatibility has been evaluated in vitro in the preosteoblastic cell line MC3T3-E1, showing adequate cell viability In addition, the effect of Cu2+ on VEGF expression on HUVEC cell line shows an increase in cell proliferation and a higher expression of VEGFR2
[1] González, B., Colilla, M., Díez, J., Pedraza, D., Guembe, M., Izquierdo-Barba, I., & Vallet-Regí, M. (2018). Acta Biomaterialia, 68, 26. [2] Mankhambo, L. A., Banda, D. L., Jeffers, G., White, S. A., Balmer, P., Nkhoma, S., Carrol, E. D. (2010). Critical Care, 14(3), 1. [3] Xie, H., & Kang, Y. J. (2009). Current Medicinal Chemistry, 16(10), 1304.