Speaker
Description
Introduction
The application of nanoCT for biological sample screening, however, remains limited due to very low intrinsic contrast of soft tissue, which means that soft tissues does not appear on regular X-ray. However, the utilization of different contrast agents can aid in visualize such soft tissue structures. Here we will present some recent advances in X-ray enhancement agents used on teeth and hydrogels, which has enabled us to visualize and quantify both soft and cellular tissues. The presentation will show that with our protocols it is possible to visualize cells alongside with mineralised tissues.to minimal speculation about the significance of the work.
Methodology: PTA in various concentrations and immersion time was tested and scanned with high resolution nano-CT. The method was applied to freshly extracted teeth where we examined both cementum and pulpal region.
Results: Three-dimensional nano-CT imaging of dental cementum and periodontium as well as interior components, such as odontoblasts and predentine, with high resolutions was made visible when using PTA staining. The thickness of the cementum could be computed over the height of the tooth made possible by the PTA-enhanced contrast, and the attached soft tissue components of the interior of the tooth could be shown on the dentine-pulp interface in great detail.
Conclusion: The developed methodology show that it is possible to visualise hard tissue along with cellular structure and soft tissues using laboratory based nano-CT technique.
| Speaker Country | Norway |
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